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qTOWER³ Real-Time PCR Thermal Cyclers

…simple Ct-value determination to ddCt-method and multi-plate analysis Vision, Amazement, Simplicity: the new qTOWER 3 real-time PCR thermal cycler from Analytik Jena. The patented fiber optic shuttle system with unique light source powered by 4 high-performance LED’s guarantees ideal…

Analytik Jena

qTOWER³ Real-Time PCR Thermal Cyclers

qTOWER3 - Get in touch with high-class qPCR

  • Patented fiber-optic system for ideal illumination and excitation of all 96 probes without edge effects
  • Minimal scan times of 6 seconds for up to 6-fold multiplexing
  • Optional filter configuration of 12 high-resolution, retrofittable color or FRET modules
  • Novel light source containing 4 long-life LEDs without pre-warm time
  • Innovative silver block with ramping rates up to 8 °C/sec and excellent temperature homogeneity
  • Optional gradient function, including linear gradient tool programming
  • Standalone operation via integrated tablet control (10") and/or comprehensive PC control
  • Use of different optical plastic materials in 0.2 mL format
  • Intuitive, multilingual control and evaluation software
  • Comprehensive portfolio of evaluation algorithms ranging from a simple Ct-value determination to ddCt-method and multi-plate analysis

Vision, Amazement, Simplicity: the new qTOWER3 real-time PCR thermal cycler from Analytik Jena. The patented fiber optic shuttle system with unique light source powered by 4 high-performance LED’s guarantees ideal excitation and detection of known fluorescent dyes up to the dark red. In addition, the system’s highly sensitive detection module can be equipped with up to 6 different color modules. Color modules are upgradeable, allowing the system to benefit from future Analytik Jena developments. And, enjoy peace of mind knowing that the qTOWER3’s optical components are backed by an industry-leading 10-year warranty. Silver block technology is at the heart of the qTOWER3, offering outstanding temperature control accuracy of ±0.1°C over the entire 96 well block. Optionally, the linear gradient function is the optimal tool to easily adjust the instrument to new assays. The qTOWERis available either as stand-alone device with integrated 10" tablet or as PC-based system. The software comes with a broad spectrum of optimized analysis algorithms including absolute and relative quantification, melt point analysis, ddCt method, PCR efficiency, allelic discrimination, endpoint and protein determination.

Patented fiber optic shuttle system

In the field of quantitative real-time PCR a wide variety of fluorescent dyes with different characteristics are applicable. In order to optimally excite each of these dyes over a wide spectral range, a novel light source with four long-life LEDs in red, green, blue and white (RGBW) is used in qTOWER3. The RGBW LED ensures best possible quantum yield of each dye in real-time PCR experiment. Highly sophisticated multiplex applications with up to 6 fluorescent-labeled probes from the blue to the near-infrared range are no challenge anymore. 

Fiber Optic System: The integrated shuttle system with 8 optical-fibers ensures an extremely fast read-out of the entire 96 well plate in only 6 seconds - irrespective of the number of dyes to be measured.

In addition, all components of the high-performance optics come with a 10-year long-term guarantee.

Maximum flexibility

Depending on the application qTOWER3’s Filter Module System can be freely configured and upgraded, to both intercalating and proteinbinding dyes, as well as to hydrolysis (e.g. Taqman®) and hybridization (FRET) probes. The system is future-proof, being easily retrofitted on-site with additional Color, FRET or Protein Modules, and can thus be adapted to other fields of application at any time.

All filter modules contain an optimized combination of excitation and emission filters which, in combination with the light source, enable the ideal detection of a multitude of commercially available fluorescent dyes.

Innovative silver block technology

Basis for a reliable performance of quantitative real-time PCR in qTOWER3 is the sample block. To ensure best possible results and outstanding energy transfer into the sample, the thermal block is made of gold-coated silver serving much better physical properties than aluminum. As a result, the qTOWER3 is characterized by excellent temperature control accuracy of ±0.1°C (over 96 wells) and first-class heating rates of up to 8°C/sec. This combination makes the overall system the ultimate for any real-time PCR application.

To guarantee the highest specificities for different assays, the device is available with a gradient function of up to 40°C spread over 12 columns. Rather then defining upper and lower temperatures as limits, the Linear Gradient Tool (LGT) allows to define simply the temperature increments as integer around the calcu-lated annealing temperature of primers in a linear fashion thus making operation of the qTOWER3 simple and intuitive.

To avoid condensation and the resulting loss of samples, the system is equipped with a motorized heated lid. This can be variably set up to 110°C and guarantees, regardless of the con-sumables used an optimal contact pressure on the sample vessels during the entire real-time PCR run.

Intuitive operation and automatic evaluation

The control and evaluation software qPCRsoft follows the concept that it should be flexible and user friendly. The logical arrangement of all tools, the intuitive handling and, last but not least, the parameter-oriented memory and programming design make the use of the software simple and clear. During an actual run, the data from previous experiments can be evaluated in parallel without complication.

Get in touch with qTOWER3

The outstanding performance of the qTOWER3 can be optionally expanded with the aid of a through-and-through innovative operation concept. The qTOWER3 touch is a modern stand-alone system which renders the need of an external control system unnecessary. The integrated 10" tablet leaves nothing to be desired with regard to experiment planning and implementation. The touch operation provides the user with a simple, intuitive menu navigation and both cycler programming as well as online monitoring and final Ct-value determination. The data files are compatible at all times with the comprehensive PC software and easily allow following evaluations.

Regardless of whether it is qTOWER3 or qTOWER3 touch - the real-time PCR thermal cyclers from Analytik Jena are the ideal and reliable tool for everyday lab work.

Thermal block
Sample blockSilver sample block with gold-coating
Block capacity96 Well á 0.2 ml for 96 x 0.2 ml
Sample size10 – 80 μl
HeatingUp to 8°C/sec (max.)
CoolingUp to 6°C/sec (max.)
Adjustable temperature range3°C to 99°C
Temperature uniformity55°C ± 0.15°C after 15 sec
Temperature control accuracy± 0.1°C
GradientLinear Gradient Tool
Max./Min. Gradient40°C / 0.1°C
Heated lid
Lid temperature30°C to 110°C
Contact pressure10 kg, automated
qPCR application
SensitivityDetects 1 copy of target sequence in human genomic DNA
Dynamic range10 orders of magnitude
Measuring principleFiber optic shuttle system with 8 fold scanner and color modules for excitation and emission filters
Light source4 longlife, high-power LED’s (RGBW)
DetectorHigh sensitive CPM (Channel Photo Multiplier)
Color modules
  • 12 Color-, FRET- and Protein Modules
  • 6 positions inside device
Read out time6 sec for 96 wells independent of the number of dyes
ControlPC or stand-alone (optional)
DisplayOnly qTOWER³ touch: 10" tablet, colored, touch, WIN 8.1
Time inc/dec±0.1 to 240 sec/cycle
Temperature inc/dec±0.1 to 20°C/cycle
Additional technical data
WeightApprox. 30 kg
Dimension (W x H x L)275 mm x 585 mm x 275 mm
  • PC connection: USB, RS232, Ethernet
  • Tablet: USB for data transfer, barcode reader
Noise emission45 db
  • 2 warranty on device system
  • 10 years long-term warranty of high performance optics

Parameters: Color Modules
NameExcitationEmissionDyes (examples)*
Color module 1470 nm520 nmFAM™, Sybr®Green, Alexa488®
Color module 2515 nm545 nmJOE™, HEX™, VIC®, YakimaYellow™
Color module 3535 nm580 nmTAMRA™, DFO™, Alexa546®, NED™
Color module 4565 nm605 nmROX™, TexasRed®, Cy3.5®
Color module 5630 nm670 nmCy5®, Alexa633®, Quasar670™
Color module 6660 nm705 nmCy5.5®, LightCycler Red®
FRET 1470 nm580 nmFAM™ (donor) / TAMRA™ (acceptor)
FRET 2470 nm670 nmFAM™ (donor) / Cy5® (acceptor)
FRET 3470 nm705 nmFAM™ (donor) / Cy5.5® (acceptor)
FRET 4515 nm670 nmJOE™ (donor) / Cy5® (acceptor)
FRET 5470 nm605 nmFAM™ (Donor) / ROX™ (acceptor)
Color module Protein 1490 nm580 nmSYPRO® Orange
* Color or FRET modules can be ordered separately.


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