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PRIMEPRO48 Real Time qPCR Thermal Cycler

…new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and f

Techne (Bibby Scientific US)

PRIMEPRO48 Real Time qPCR Thermal Cycler

The Prime Pro 48 real time PCR system is a new, high specification, economically priced real time thermal cyclerfrom Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.

High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimised 40 cycle reaction down to only 15 minutes. PCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.

The capacity of Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity.

Key Benefits

Speed

  • 40 cycles in 40 minutes before optimisation
  • 40 cycles in 15 minutes when optimised
  • Genotype in 4 minutes with over 99% accuracy
  • Set up a 4plex reaction in only 4 mouse clicks

Confidence

  • MIQE compliant - always consistent, always compliant
  • Instant settle time to achieve world leading uniformity of ±0.1°C across the block - any well, any instrument - consistent results in any system
  • 400 analyte specific qPCR reagent kits available
  • Demo equipment available to evaluate in your own lab

Performance

  • Consistent results provide trust and confidence. Well-to-well, run-to-run , machine-to-machine
  • Compile data from multiple experiments. Pool plates and results across labs and instruments
  • Record every well, with every filter, in every cycle. Never miss a data point.
  • Adaptive LED Control means you never have uncertainty of saturated unknowns, no bleaching though light to neighbouring wells or maximising the wide dynamic range
  • Trust results are correct
  • All other block-based qPCR machines have lower uniformity
  • If you perform Next Generation Sequencing, any other instrument than Prime Pro 48 is risking your NGS, with significant cost implications

Value

  • No other qPCR system offers so much performance for such a low price
  • Available as a complete system - Techne instrument, plates, seals and reagents
  • Alternatively, is chemically open platform - use your preferred supplier reagents
  • Lowest price per run - use duplicates and low sample volumes. Don't waste money with triplicates and large sample volumes.
  • Everything as standard - no add-ons, HRM as standard, unlimited software licence
  • Highly accurate and uniform qPCR prevents means successful NGS runs and significant moneysaving

Sensitivity

  • ±0.1°C uniformity across the whole block instantly after every temperature change means that any well, in any instrument, provides same answer
  • HRM - Identify Class IV SNP >99.9% of the time
  • Unsurpassed block uniformity with patented block technology enables the researcher to use duplicates on Prime Pro 48, whereas triplicates are required on less uniform competitor machines

Volume per wellValidated for 5 to 20 ul
Detection sensitivity1 copy
Temp uniformity+- 0.1°C within 1 sec
Temp stability+- 0.1°C
Temperature range30 to 100°C
Average ramp rate5.5°C sec
Thermal systemHollow silver block peltier-based with conductive fluid
Block format48-well block
Consumables48-well custom plates and optical adhesive seals
Optical systemDual LED excitation (452 to 486 nm and 542 to 582 nm)
CameraCCD camera
Filters4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)
Calibrated dyesSYBR FAM HEX ROX Cy5
Additional dyesAll dyes within range compatible with no additional calibration
Passive reference dyesUse of ROX is supported but optional
Data collectionData always collected in all four filters for all wells
Plate setupPlate setup for data analysis can be altered after run completes
Melt curveSupports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
PCR cycle time (unoptimized)40 cycles in less than 40 minutes
PCR cycle time (FAST optimized)40 cycles in less than 20 minutes
Dynamic rangeGreater than 9 logs
CalibrationNot required
InstallationPlug and play design
PrecisionDiscriminates 5000 and 10000 template copies with 99 percent confidence
Warranty1 year (parts and labor included)
Voltage100 to 240 V.AC
Frequency50 and 60 Hz
Nominal current draw5A
Peak power500W (typical power is 180W)
SoftwareMultiple license software included at no additional cost
ChemistryAll chemistries supported
ApplicationsAbsolute Quantification and Relative Quantification
ApplicationsAllelic Discrimination and High Resolution Melt
Dimensions closed (WxDxH)34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)
Dimensions open (WxDxH)34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)
Weight13.6 Kg (30 lb)

 


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